![]() (2013) Lymphocyte crossmatching by flow cytometry. Cytometry B Clin Cytom 90B:177-190.īray RA. (2016) Validation of a flow cytometry-based assay to assess C5aR receptor occupancy on neutrophils and monocytes for use in drug development. Quadrini KJ, Hegelund AC, Cortes KE, Xue C, Kennelly SM, Ji H, Högerkorp C-M, Mc Closky TW. (2008) Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor agonist. SDS ST334, (QMESF AF 488, PE & APC) Catalog Number PDS 849, Determination of Effective F/P Ratio Quantum™ MESF kits are available in three sizes: A (20 tests), B (100 tests), and C (280 tests). A free QuickCal ® analysis template is provided with each kit to aid in determining the expression level of cells, and for evaluating instrument linearity and detection threshold. See PDS 849 to determine effective FP ratio. The assignment of fluorescence intensity in Molecules of Soluble Fluorochrome (MESF) units is performed through direct comparison of fluorescence measurements from solutions of the pure fluorochrome with those from microspheres surface-labeled with the same fluorochrome. Our Quantum™ MESF kits contain one blank population and a series of either four fluorescent microsphere populations labeled with varying amounts of Alexa Fluor ® 488, Alexa Fluor ® 647, APC, or R-PE, or five fluorescent microsphere populations labeled with varying amounts of FITC. quantitation of the fluor signal from each cell population).įor additional information about working with these products, see our Quantitative Cytometry brochure and our Tips & Troubleshooting PDS or check out our Flow Blog. Cell samples are then read against the curve for determination of expression (i.e. To use, beads are run on the same day and at the same PMT & compensation settings as samples to establish a calibration curve that relates instrument channel values and standardized fluorescence intensity units (MESF or ABC). Because they are labeled with the same fluorochromes used to label cells, they provide a synchronous response to the environment. ![]() Our Quantum™ MESF and Quantum™ Simply Cellular ® beads are external standards that enable the standardization of fluorescence intensity units irrespective of cytometer and software.
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